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Image Search Results
Journal: Cell Death & Disease
Article Title: mtDNA-STING pathway promotes necroptosis-dependent enterocyte injury in intestinal ischemia reperfusion
doi: 10.1038/s41419-020-03239-6
Figure Lengend Snippet: ( A – C ) IHC analysis of p-RIPK3 and p-MLKL in wild-type mice and STING knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: The PVDF membranes were incubated overnight at 4°C with primary antibodies, including MLKL (A5579, Abclonal), p-MLKL (phospho S345, ab196436, Abcam), RIPK3 (17563-1-AP, Proteintech), p-RIPK3 (phospho T231 + S232, ab222320, Abcam),
Techniques: Paraffin-embedded Immunohistochemistry, Knock-Out, TUNEL Assay, Staining, Immunohistochemistry, Western Blot