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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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( A – C ) IHC analysis <t>of</t> <t>p-RIPK3</t> and p-MLKL in wild-type mice and <t>STING</t> knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Image Search Results


( A – C ) IHC analysis of p-RIPK3 and p-MLKL in wild-type mice and STING knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Cell Death & Disease

Article Title: mtDNA-STING pathway promotes necroptosis-dependent enterocyte injury in intestinal ischemia reperfusion

doi: 10.1038/s41419-020-03239-6

Figure Lengend Snippet: ( A – C ) IHC analysis of p-RIPK3 and p-MLKL in wild-type mice and STING knockout mice following intestinal I/R. TUNEL staining was used to analyze cell death. Image J was used to detect the optical density of p-RIPK3 and p-MLKL in immunohistochemistry. ( B ) Necroptosis signaling was detected by western blot. Scale bars = 50 μm. Data were shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The PVDF membranes were incubated overnight at 4°C with primary antibodies, including MLKL (A5579, Abclonal), p-MLKL (phospho S345, ab196436, Abcam), RIPK3 (17563-1-AP, Proteintech), p-RIPK3 (phospho T231 + S232, ab222320, Abcam), STING (19851-1-AP, Proteintech), TBK1 (38066, Cell Signaling), p-TBK1 (phospho S172, 5483, Cell Signaling), IRF3 (4302, Cell Signaling), p-IRF3 (phospho S396, 29047, Cell Signaling), P65 (A2547, Abclonal), p-P65 (phospho S536, AP0475, Abclonal) and β-actin (4970, Cell Signaling).

Techniques: Paraffin-embedded Immunohistochemistry, Knock-Out, TUNEL Assay, Staining, Immunohistochemistry, Western Blot